[PEER REVIEWED] Preparation of samples for Lipid-CLEM imaging v1
Abstract
The Lipid-CLEM method enables room temperature correlative light and electron microscopy visualization of the intracellular localization of lipids. It combines minimally modified (bifunctional) lipid probes and on-section click chemistry labelling. Using Lipid-CLEM, relative lipid densities in membrane nanodomains can be determined. This protocol covers the essential aspects for monitoring lipid localization by CLEM.
Fields of Study & Subjects
Lipid Membrane Structure and Behavior
Advanced Electron Microscopy Techniques and Applications
Advanced Fluorescence Microscopy Techniques
Publication details
- Publisher
- Springer Science and Business Media LLC
- Publication date
- 9 February 2026
- Document type
- Preprint