Protoplast and PEG-mediated transformation of Zymoseptoria tritici v1

2026 Preprint Open access

Read full text
Authors: Michael Choi, Megan C. McDonald
DOI: 10.17504/protocols.io.6qpvrbenblmk/v1
Cited by: 0

Abstract

This is a protocol for generating competent protoplasts from the fungal wheat pathogen Zymoseptoria tritici using an affordable commercial enzymes, Extralyse/Vinotaste. This protocol uses PEG-mediated transformation to ectopically integrate linear DNA (PCR-products) into the genome.

Fields of Study & Subjects

Fungal and yeast genetics research Plant Pathogens and Fungal Diseases Wheat and Barley Genetics and Pathology

Publication details

Publication date
9 March 2026
Language
EN
Document type
Preprint