WildinSync - eDNA laboratory extraction v1

This protocol is used in the context of the long-term global biodiversity monitoring initiative WildinSync, which applies environmental DNA (eDNA) metabarcoding to assess anthropogenic impacts and nature-positive actions on ecosystems. The filtration of eDNA from water bodies provides a non-invasive approach to assess biodiversity across marine and terrestrial systems. This protocol describes the processing of water filtrates conserved in a buffer followed by DNA extraction and purification. The extracted DNA is intended for downstream molecular analyses, including qPCR and metabarcoding, to enable biodiversity monitoring.