Cell lysis for preparation of protein extracts for quantitative proteomics - Kryptoperidinium foliaceum, Nitzschia laevis and Symbiodinium sp. v1
Abstract
This methodology was developed for the lysis of dinoflagellates and diatoms with tough cell walls, using glass-bead sonication in a lysis buffer containing concentrated urea and detergent. This was necessary for strains where sonication without the presence of glass beads was unable to lyse cells.
Fields of Study & Subjects
Advanced Proteomics Techniques and Applications
Protist diversity and phylogeny
Marine Plankton and Biogeochemistry
Publication details
- Publication date
- 3 February 2026
- Language
- EN
- Document type
- Preprint