Ngn2 differentiation protocol for iPSCs without Ngn2 transgene knock-in v1

2026 Preprint Open access

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Authors: Dahlia Rohm, Joshua B. Black, Sean McCutcheon, Alejandro Barrera, Shante Berry, Daniel Morone, Xander Nuttle, Celine E. de Esch, Derek Tai, Michael E. Talkowski, Nahid Iglesias, Charles Gersbach
DOI: 10.17504/protocols.io.81wgbowpnlpk/v1
Cited by: 0

Abstract

This protocol describes methods for generating excitatory neurons from iPSCs using transduction of virus expressing inducible neurogenin-2 (Ngn2).

Fields of Study & Subjects

Pluripotent Stem Cells Research Neurogenesis and neuroplasticity mechanisms Developmental Biology and Gene Regulation

Publication details

Publication date
2 March 2026
Language
EN
Document type
Preprint