Senescence induction by ionizing radiation in human PCLS v1

Precision-cut lung slices (PCLS) are standardized human lung tissue sections that serve as an advanced ex vivo platform for studying disease mechanisms, screening therapeutic compounds, and supporting preclinical research, while preserving native cellular diversity and structural organization of the lung. This protocol outlines an method for inducing cellular senescence in PCLS using ionizing radiation, along with procedures for harvesting tissue samples for downstream analyses and quantitative assessments.