Hematoxylin 26 Eosin Staining of Clinical Tissues in Formalin Fixed Paraffin Embedded (FFPE) Samples v1

This stain is utilized when evaluating tissue architecture and cellular morphology in histological samples. Hematoxylin and Eosin (H26E) staining helps not only in identifying different cell and tissue types, but also in visualizing nuclear and cytoplasmic components with high structural detail. This protocol describes the stepwise process for staining clinical FFPE samples to visualize cellular and extracellular structures using the histological dyes hematoxylin and eosin. Hematoxylin stains ribosomes and various other nuclear structures a deep blue-purple, while eosin stains the cytoplasm, collagen, connective tissue, and surrounding extracellular matrix in orange-pink to red hues. This method clearly demonstrates a broad range of cytoplasmic, nuclear, and extracellular matrix features, making H26E great for pathologists.