ddPCR Protocol: Detection and Quantification of Integrated HIV Psi Sequence v1

2026 Preprint Open access

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Authors: Hayley Todesco, Rebecca J. Malott, Kyle Potts, Douglas Mahoney
DOI: 10.17504/protocols.io.bp2l6ok9klqe/v1
Cited by: 1

Abstract

Detection and quantification of the HIV-1 Psi sequence integrated into cells following lentiviral (LV) transduction. Performed as singleplex reactions for targeting Psi or the reference gene RPP30 using the Bio-Rad QX600™ Droplet Digital PCR (ddPCR)™ system with AutoDG following manufacturer instructions.

Fields of Study & Subjects

HIV Research and Treatment Virus-based gene therapy research Cancer-related Molecular Pathways

Publication details

Publisher
Springer Science and Business Media LLC
Publication date
27 May 2026
Document type
Preprint