Visium HD Spatial Gene Expression Protocol: Library Preparation, Sequencing, and Analysis v1

2026 Preprint Open access

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Authors: Gurveer Gill, Joanna Pyczek, Bo Young Ahn, Jennifer Chan, A. Sorana Morrissy
DOI: 10.17504/protocols.io.bp2l6okd1lqe/v1
Cited by: 1

Abstract

High-resolution spatial transcriptomic profiling of formalin-fixed, paraffin-embedded (FFPE) tissue sections using the 10x Genomics Visium HD platform. This includes a custom GCAR1 probe panel addon (12 spike-in probes) for detection of the GCAR1 construct in ASPS xenograft and biopsy samples.

Fields of Study & Subjects

Single-cell and spatial transcriptomics Molecular Biology Techniques and Applications Gene expression and cancer classification

Publication details

Publisher
Springer Science and Business Media LLC
Publication date
28 May 2026
Document type
Preprint