4.5x Expansion of Mouse Brain tissue sections v1

This technique expands mouse brain tissue 4.5-fold in all spatial dimensions by embedding brain slices in swellable acrylamide gels. This allows enhanced resolution compared to traditional microscopy to precisely investigate subcellular targets in the brain. Here we adapted previously developed expansion protocols to reliably image enlarged Arl13b-GFP-positive cilia of striatal cholinergic interneurons in Arl13b-GFP knock-in mouse brain slices.