A Surgical Protocol for Precise High-Throughput and Precise Viral Injections in Rhesus Monkey Brain v1

Viral injections in nonhuman primates allow modification of neurons to investigate the organization and function of neural circuits, their developmental processes, and disease-related mechanisms. Here we present a step-by-step protocol for performing precise stereotaxic microinjections in the macaque brain. This protocol covers the entire workflow, from pre-surgical imaging and target planning to perioperative care and detailed surgical procedures for multiple subcortical and cortical regions. For complete details on the use and execution of this protocol, please refer to Furlanis et al.1.