Dried plasma spot biobanking protocol v1

This protocol presents methods for collection and biobanking of plasma in the form of a dried plasma spot (DPS) from whole blood drawn by standard phlebotomy. Leveraging Capitainer SEP10 cards, for a shelf stable, consistent 10 ul DPS, this protocol may be inserted in the beginning of any workflow involving fresh anticoagulated whole blood, such as isolation of peripheral blood mononuclear cells (PBMC), in which only 75-150 ul may be available to spare. Resulting DPS are held at room temperature until elution for downstream applications enabling a more flexible workflow compared to standard plasma biobanking methods. Accurate quantification of analytes for proteomic assessment may be achieved by correcting through a dilution factor, considering (i) phlebotomy tube anticoagulant volume and (ii) total volume whole blood drawn. This protocol supplements original work published by Capitainer AB, Uppsala, Sweden.