Measuring cGAMP production in living single cells using a dedicated FRET reporter v1

This protocol outlines a workflow to generate, validate and image cell lines stably expressing a cGAMP FRET reporter in order to measure cGAMP production in live single cell assays. We detail how to generate lentiviral particles, transduce MCF10A cell lines and validate the most appropriate clones using FRET live single cell imaging. Finally, we provide advice on how to best image our cGAMP FRET reporter despite low levels of expression and challenging imaging conditions.