BCRseq Analysis using Dandelion v1

Naive B cells amplify and diversify their responses when activated by cognate antigen, via clonal expansion, immunoglobulin isotype switching, phenotypic variation, and somatic hypermutation (SHM). Since diversity in the antigenic targets, functional classes, and production kinetics of parasite-specific antibodies influences immunity to malaria, we test here whether individual B cell clones diversify over time in vivo during Plasmodium infection and treatment. Here we share our step by step protocol for analysing BCRseq data using Dandelion.