IHC Protocol: Immunohistochemical Staining of Paraffin-Embedded Tissue Sections for GPNMB, TFE3, PD-L1, CD3, MYC, and Ki67 v1

Franz Zemp; Liu Hongrui; Douglas Mahoney

doi:10.17504/protocols.io.ewov1ejnpgr2/v1

IHC Protocol: Immunohistochemical Staining of Paraffin-Embedded Tissue Sections for GPNMB, TFE3, PD-L1, CD3, MYC, and Ki67 v1

2026 Preprint Open access

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Authors: Franz Zemp, Liu Hongrui, Douglas Mahoney

DOI: 10.17504/protocols.io.ewov1ejnpgr2/v1

Cited by: 1

Abstract

Immunohistochemical (IHC) detection and visualization of target proteins in formalin-fixed, paraffin-embedded (FFPE) tissue sections using horseradish peroxidase (HRP)-based detection with 3,3’-diaminobenzidine (DAB) chromogen. This protocol is optimized for the following primary antibodies: anti-human GPNMB, TFE3, PD-L1, CD3, MYC, and Ki67.

Fields of Study & Subjects

HER2/EGFR in Cancer Research Peptidase Inhibition and Analysis Molecular Biology Techniques and Applications

Publication details

Publication date: 27 May 2026
Document type: Preprint