Endotracheal Wounding Using Derby Perry Excavator in Wistar Rats v1

In vivo endotracheal wounding in Wistar rats presents notable technical challenges. Proper animal positioning and adequate illumination are necessary for clear visualization of the glottis and trachea, while selecting an appropriate instrument is critical to avoid perforation or insufficient injury. Although Hillel et al. previously described a wire-brush technique in mice, limited access to this tool motivated the development of alternative approaches. Utilization of a pen light with an acrylic attachment and modified Derby Perry dental excavator provided optimal visualization and generated consistent, controlled partial-thickness wounds. This approach proved to be the most effective technique for producing endotracheal injuries in Wistarrats.