Toxicity Testing of Compounds in Galleria mellonella Larvae v1

This protocol describes a method for toxicity screening of chemical compounds, nanoparticles, or microbial metabolites using Galleria mellonella larvae. The procedure utilizes 7th instar larvae injected with test compounds via the last proleg, followed by survival monitoring over 72 hours. With a sample size of 10+ larvae per treatment group, this assay allows for statistically strong, rapid, low-cost preliminary toxicity screening in an invertebrate model that shares key physiological similarities with mammals.