Immunostaining v1

This immunohistochemistry protocol was developed to visualize pathological markers in AOF-PD tissue, focusing on tyrosine hydroxylase (TH), microglial marker Iba1, GFAP, and DAT with quenching of endogenous peroxidases to reduce background. The protocol enables multiplexed fluorescent detection of dopaminergic neurons, microglia, and pathological alpha-synuclein aggregates, facilitating the study of neuroinflammatory and neurodegenerative features in Parkinsonian models.