tRIBO-seq: Selective profiling of translationally active tRNAs v2

Here we describetRIBO-seq, a simple and robust nanopore-based method for the selective capture of ribosome-associated native tRNA populations (ribo-tRNAs) from actively translating ribosomes. This approach enables the simultaneous assessment of tRNA abundance, modification status, and fragmentation within a single experiment. The protocol details the recovery of ribosome-embedded tRNAs, which can subsequently be sequenced using nanopore or Illumina platforms.