PacBio Ampli-Fi - Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing v1

This is a modified protocol for preparing PacBio SMRTbell libraries with Prep kit 3.0 of ultra low input DNA samples. The protocol is based on Ampli-Fi - Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing (103-648-000 REV04 JUL2025). The main differences are: adding MgCl2 and changing incubation times at adapter ligation changing reagent amounts and incubation times at nuclease treatment