Immunofluorescence (IF) Staining Technology v1

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Authors: Peizheng Shi
Cited by: 0

Abstract

Immunofluorescence (IF) is an optical imaging sample preparation technique widely utilized in biomedical and cell biology research. It enables the detection and localization of specific proteins within cells or tissue sections using fluorophore-labeled antibodies. By combining the high specificity of antigen-antibody recognition with fluorescent labeling, researchers can visualize the distribution and relative abundance of target proteins under a fluorescence microscope. When integrated with advanced imaging modalities such as super-resolution microscopy or Fluorescence Lifetime Imaging Microscopy (FLIM), IF provides exceptionally detailed and intuitive insights into cellular architecture.

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Cell Image Analysis Techniques Spectroscopy Techniques in Biomedical and Chemical Research

Publication details

Publication date
12 February 2026
Language
EN
Document type
Preprint