Ex Vivo Whole-Cell Patch Clamp Recording and Pharmacological Isolation of Light-Evoked GABAA Currents v1

2026 Preprint Open access

Read full text
Authors: Nicola Berretta, Ezzia Guatteo, Nicola Biagio Mercuri
DOI: 10.17504/protocols.io.kxygx82odv8j/v1
Cited by: 0

Abstract

This protocol describes whole-cell patch clamp recordings from VTA neurons in acute horizontal slices. Light-evoked synaptic currents are recorded at -60 mV and pharmacologically isolated. GABAA receptor mediation is confirmed by picrotoxin sensitivity.

Fields of Study & Subjects

Neuroscience and Neuropharmacology Research Neurotransmitter Receptor Influence on Behavior Neural dynamics and brain function

Publication details

Publication date
16 February 2026
Language
EN
Document type
Preprint