HiDEF-seq v2

2026 Preprint Open access

Read full text
Authors: Nisrine T. Jabara, Mei Hong Liu, Benjamin M. Costa, Emilia C. Bianchini, Una Choi, Rachel C. Bandler, Emilie Lassen, Marta Grońska-Pęski, Adam Schwing, Zachary R. Murphy, Daniel Rosenkjær, Shany Picciotto
+13 more Vanessa Bianchi, Lucie K. Stengs, Melissa Edwards, Nuno Miguel Nunes, Caitlin A. Loh, Tina K. Truong, Randall E. Brand, Tomi Pastinen, J. Richard Wagner, Anne-Bine Skytte, Uri Tabori, Jonathan E. Shoag, Gilad D. Evrony
DOI: 10.17504/protocols.io.kxygxy9mwl8j/v4
Cited by: 0

Abstract

This is the HiDEF-seq v3.1 library preparation protocol for bulk, single-molecule fidelity, long-read sequencing. This version of the HiDEF-seq protocol is designed for whole-genome profiling via random fragmentation and larger fragments than in our prior protocol for increased cost-efficiency. See our paper "DNA mismatch and damage patterns revealed by single-molecule sequencing" (Liu & Costa et al.) for more information.

Fields of Study & Subjects

Genomics and Phylogenetic Studies Single-cell and spatial transcriptomics DNA Repair Mechanisms

Publication details

Publication date
9 January 2026
Document type
Preprint