UMN-TMCs: CosMx Spatial Molecular Imager for FFPE Tissue – Protein 64-Plex (v1) v1

The CosMx Spatial Molecular Imager (SMI) enables high-plex in situ visualization of individual RNA transcripts and/or protein stain overlays with 120 nm spatial resolution. This protocol outlines basic criteria for sample preparation or selection; tissue slide processing; and imaging to generate 64-plex CosMx Protein data and export data from the AtoMx Spatial Informatics Platform (SIP). The steps herein – based largely on materials developed by and property of Bruker Spatial Biology, Inc. – are specific procedures followed by the University of Minnesota Tissue Mapping Centers (TMCs) as part of the NIH Common Fund's SenNet Consortium and are not officially condoned by Bruker Spatial Biology.