GeoMx Whole Transcriptome Atlas (WTA) Assay with H&E-Guided ROI Selection for FFPE Human Lung Tissue Microarrays (TMA) v1

This protocol describes the workflow for performing the NanoString GeoMx Digital Spatial Profiler Whole Transcriptome Atlas (WTA) assay on FFPE human lung TMA samples using: DAPI as the sole morphology marker H&E staining on serial sections for structural guidance GeoMx FFPE Slide Prep Kit (Buffer R/S) for RNA target retrieval Standard NanoString WTA probe hybridization, stringent washes, UV cleavage, NGS library preparation, and sequencing This version is optimized for normal human lung tissue microarrays (TMAs), in which morphology may not be fully resolved with DAPI staining alone, and accurate ROI placement is achieved through virtual overlay of H&E-stained serial sections.