WildinSync-eDNA sampling of rivers v1

This protocol was developed to support long-term global biodiversity monitoring under the WildinSync initiative, which uses environmental DNA (eDNA) metabarcoding to assess the effects of nature-positive actions on ecosystems. Freshwater sampling is performed to capture DNA traces left by plants or animals, enabling detection of species presence and distribution across diverse habitats. The method consists of standardized Freshwater eDNA sampling to obtain DNA traces from aquatic animals. It provides guidance on required materials, field sampling steps, and preparation of samples for later molecular analyses (e.g., DNA extraction and sequencing). The protocol is applicable across a wide range of habitat types and environmental conditions worldwide. Applicability may be limited in environments where water sampling is not feasible or safe.