Single-Cell GEX and VDJ (TCR) Sequencing Protocol: Library Preparation, Sequencing, and Analysis v1

Comprehensive single-cell transcriptomic (GEX) and T-cell receptor (TCR/VDJ) profiling of peripheral blood mononuclear cells (PBMCs) using the 10x Genomics Chromium GEM-X platform. The workflow includes library preparation, sequencing, TCR clonotype assignment, and downstream scRNA-seq analysis with custom reference (human genome + GCAR1 construct), quality control, integration, clustering, cell-type annotation via SCimilarity, GCAR1 status evaluation, and marker gene profiling.