From stem cells to stem cell-free therapies: An umbrella review of regenerative approaches in wound healing

This project contains the protocol, data extraction materials, and analytic outputs for an umbrella review synthesizing evidence from systematic reviews and meta-analyses on regenerative approaches for skin wound healing. The review evaluates both stem cell–based therapies (including autologous skin cell suspensions, mesenchymal stem/stromal cells, and biologic skin substitutes) and stem cell-free strategies, particularly mesenchymal stem cell–derived extracellular vesicles. Methodological quality is assessed using AMSTAR-2, and overlap of primary studies across included meta-analyses is quantified using the Corrected Covered Area (CCA). The project aims to provide a high-level, methodologically rigorous synthesis to inform clinical practice, translational research, and policy development in regenerative wound management.