High-Throughput Approaches to Understanding Microbial Phosphate Solubilization for Sustainable Agriculture: A Systematic Review

This study aims to systematically review how high-throughput omics approaches have advanced the identification and understanding of phosphate-solubilizing microorganisms in agricultural systems over the past decade. Although high throughput technologies such as 16S rRNA metabarcoding, amplicon sequencing, metagenomics, whole-genome sequencing, transcriptomics, and proteomics are increasingly used to study microbial phosphate solubilization, a consolidated systematic synthesis of this literature is currently lacking. The review will synthesize existing evidence to evaluate a decade (2015-2025) methodological trends, key findings, and knowledge gaps, with the expected outcome of providing a comprehensive overview to support future research in phosphate solubilizing microorganisms.