VDAC1 Gate-Opening Therapeutic Stack for MSS Colorectal Cancer: From Gate-Jamming Score to Gate-Opening Sequence

Strategic Architecture Document (SAD v4.0) reformulating the VDAC1 gate-opening therapeutic hypothesis for microsatellite-stable (MSS) colorectal cancer — a tumor subtype that is invisible to checkpoint inhibitors. The repository details how a three-phase original hypothesis (TSPO inhibition → immune activation → CBD apoptosis) was falsified by its own literature review, yielding a mechanistically cleaner surviving stack: Lovastatin (cholesterol lock) → VDAC1 oligomerization → mtDNA release → cGAS-STING activation → Botensilimab-amplified innate/adaptive immune response. Includes six critical experiments with explicit kill conditions, a complete bench protocol for the linchpin lovastatin experiment (HCT116, $3,010 budget, 4 weeks), the Gate-Jamming Score (GJS) cofactor equation, a 9-month execution timeline (Q2–Q4 2026), and a TNBC extension hypothesis. Author: Anthony J. Vasquez Sr., Delaware Valley University. License: CC BY-NC 4.0. GitHub: https://github.com/templetwo/VDAC1-Gate-Opening-Therapeutic-Stack