Protective Mechanism of Jiuni Jiedu Decoction against Sepsis-Induced Cardiomyopathy: A Study Integrating Network Pharmacology and Experimental Validation

We employed network pharmacology to predict the active components, targets, and pathways ofJNJDD in sepsis. A cecal ligation and puncture (CLP) mouse model of SIC was established. Cardiac function was assessed via echocardiography, myocardial injury by histopathology (H&E staining) and serum cardiac troponin I (cTnI) levels. Inflammatory cytokines and apoptosis-related proteins were evaluated using ELISA, Western blot, and PCR.Network analysis identified 198 active components and 2,667 potential targets ofJNJDD, with 117 targets overlapping with sepsis. In vivo,JNJDD administration improved cardiac function, reduced myocardial injury marker (cTnI), attenuated pathological damage, and decreased levels of inflammatory factors and apoptosis-related proteins in CLP-induced mice.