G. mellonella_TimeDependent_Proteome

Thermal variation significantly affects insect physiology, cellular balance, and stress resilience. In this study, we conducted a comparative proteomic analysis of Galleria mellonella larvae exposed to three temperatures (18, 30, and 37 °C) for 24 and 48 hours to understand the molecular responses over time to thermal stress. Twodimensional gel electrophoresis followed by LC–MS/MS identified distinct sets of proteins uniquely expressed at each temperature–time combination, as well as a core group consistently present across treatments.