scRNA-seq: Effect of age, lysosomal degradation and mitochondrial replication on mouse hematopoietic stem cell

Mouse hematopoietic stem cell (HSC) were isolated from bone marrow by FACS sorting of lineage negative (Lin-) Sca1+ cKit+ (LSK) CD150+CD48- cells from 8 weeks (young) or 20 month old (old) mice to be cultured 16hours ex-vivo in presence or not of ConA (40 nM) or ddc (40uM). All conditions were assessed by scRNA-seq in adddition to freshky isolated HSC from young or old mice. Note: HSC from young mice cultured 16h without drug were labelled "Old-like_mHSC_Cultured". For each samples, a barcodes.tsv, a features.tsc and a matrix.mtx are available.