PT/HS EC Spatial Transcriptomics

A murine model of severe polytrauma-hemorrhagic shock (PT/HS) was used to compare pulmonary vascular endothelial cell (EC) responses to resuscitation with lactated Ringer’s (LR) relative to fresh frozen plasma (FFP). At 24h after PT/HS or sham procedure, lungs were fixed and processed for spatial transcriptomic profiling of pulmonary arterial, venous, and microvascular ECs using the GeoMx Digital Spatial Profiler. Differentially expressed genes and pathway analysis revealed enrichment of biological processes related to cellular bioenergetics and metabolic recovery pathways in ECs after FFP, whereas LR was associated with oxidative stress and inflammatory signaling.