Molecular mechanisms of ARID5B

Raw Western Blot images from three different experiments as listed below: 1- A V5 co-IP was performed in HAP1 cells overexpressing V5-GFP, full length, and domain mutants (delta ARID and delta BAH domains) of V5-ARID5B. Input as well as IPs were blotted for HDAC1 (Cell Signaling, 5356), HDAC2 (Cell Signaling, 57156), H3 (Abcam, Ab1791), and V5 (Thermo, R960-25). 2- NALM6 WT and ARID5B KO cells were stimulated with 10µ/mg Igµ for 10min. Expression of Erk (Cell Signaling, 4696), pErk (Cell Signaling, 4370), Akt (Cell Signaling, 2920) and pAkt (Cell Signaling, 4060) was probed for. 3- Protein levels of HDAC1 (Cell Signaling, 5356), HDAC2 (Cell Signaling, 57156), H3K27ac (Active Motif, 39133), H3K4me1 (Diagenode, C15410194) and H3 (Abcam, Ab1791) were assessed in NALM6 WT and ARID5B KO cells.