Transcriptomic Analysis of Rnq1 Loss and Prionization: Effects on Translation Pathways and Energy Metabolism

Prion proteins can undergo self-perpetuating conformational changes that alter their functions. One such protein, Rnq1, is unknown for its physiological functions. We conducted RNA-seq experiments to compare the effects of Rnq1 depletion and prionization. This dataset includes summary of the sequencing data, especially information on differentially expressed genes (DEGs). Description of the data/file structure The three supplemental tables have similar data structues, containing summarized RNA-seq results generated with a standard pipeline. These files are in .CVS format and can be opened with Excel or similar software. Table S1_RM vs WT is for DEGs between Rnq1 depletion and wildtype non-prion strains; Table S2_RP vs WT is for DEGs between Rnq1 prion and wildtype strains; Table S3_RP vs RM is for Rnq1 prion and depletion strains. Variables Systematic Name: Yeast systematic gene name Standard Name: Standard gene name Allias: other name Chr: chromosome Start: gene start position End: gene end position width: Gene size in base pairs Strand: forward or complementary strand LL.RM1: Rnq1 depletion mutant sample 1 LL.RM2: Rnq1 depletion mutant sample 2 LL.RM3: Rnq1 depletion mutant sample 3 RP1: Rnq1 prion sample 1 RP2: Rnq1 prion sample 2 RP3: Rnq1 prion sample 3 LL.CK1: wildtype non-prion sample 1 LL.CK2: wildtype non-prion sample 2 LL.CK3: wildtype non-prion sample 3 meanRM: average reads of Rnq1 depletion mutant samples meanRP: average reads of Rnq1 prion samples meanWT: average reads of Wildtype non-prion samples FC: fold change logFC: log(fold change) log(adjP)abs: log value of adjusted P meanALL: average of all STDEVall: STDEV logCPM: log value of count per million LR: system value from the pipeline PValue: P value adj.p: adjusted P value up: upregulated based on the cutoff criteria dn: downregulated based on the cutoff criteria biotype: Feature type of gene description: gene function description