Chemical Profile of Ficus carica L. Leaf Methanol Extract And Its Effect On In Vitro Wound Healing

Ficus carica L. is among the earliest known cultivated plants of the family Moraceae. It constitutes one of the most important angiosperm genera, encompassing more than 800 species of shrubs, epiphytes, and trees that have been planted in several tropical and subtropical regions across the globe. Ficus carica (FC) leaves have been used not only for tea but also for traditional medicine purposes throughout history. In study, the content of Ficus carica leaf methanol extract (M-FCL) and its proliferation and cell viability properties in human dermal fibroblast (HDF) cell culture were examined. In the first stage of the study, different components in the structure of the extract were identified using gas chromatography-mass spectrometry (GC-MS) and secondly, HDF cell viability (WST-8) and wound healing activity were evaluated. HDF cell viability (WST-8) and wound healing activity were evaluated. To evaluate the wound healing effects of the extract, microscopic images were taken at three-hour intervals (0, 3, 6, 9, 12 and 15 hours) until the wound was closed in any group. Then, they were analyzed with Image J program. Fourteen chemical components were identified in the M-FCL extract by means of GC-MS. Analysis reveals that the main constituent of M-FCL is α-tocopherol (28.181 %). As a result of WST-8 analysis, it was observed that cell viability increased significantly (p < 0.01) at concentrations between 31.25-250 µg/mL. It was observed that M-FCL accelerated wound healing at low concentrations.