Investigating the Influence of Black Cumin Seed Extract on Sperm Quantity in CCl4-Rats

KnE Medicine 2026 Open access

Read full text
Authors: Fathiyah Safithri, Nayla Nugrahandini, Mochamad Aleq Sander, Desy Andari, Aji Muthi’ah Nur Azizah
DOI: 10.18502/kme.v4i1.20772
Cited by: 0
References: 18

Abstract

Background: Infertility can result from toxic-induced testicular damage leading to decreased spermatozoa. Black cumin seed extracts contains bioactive compounds (thymoquinone, oleic acid, linoleic acid) with anti-inflammatory potential that may protect spermatogenesis. Aims: To evaluate the effect of Black cumin seed extracts on spermatozoa count in CCl4-induced male rats. Methods: A true experimental, post-test only control group design was applied in 30 male rats randomized into five groups (normal, CCl4 control, and three treatment groups receiving graded doses of Black cumin seed extracts. Results: Black cumin seed extracts significantly increased spermatozoa count across groups (One Way ANOVA, p

Fields of Study & Subjects

Sperm and Testicular Function Nigella sativa pharmacological applications Medicinal Plant Research

Publication details

Journal / source
KnE Medicine
Volume
4
Issue
1
Pages
20–28
Publication date
4 March 2026
Language
EN