Mitigation of DPT Vaccine-induced Hyperthermia by Pandanus amaryllifolius Roxb Extract in Rat Models

Background: Fever is a common side effect following DPT immunization. Paracetamol prophylaxis lowers DPT vaccine response. Pandanus amaryllifolius extract (PAE) exhibits antipyretic properties due to its kaemferol and naringenin content, and it also boosts immunity through its ferulic acid content. Aims: To prove that it could reduce rectal temperature, TNF-α, and increase IL-10 in Wistar rats induced by the DPT vaccine Methods: Twenty-five Wistar rats were divided into five groups: negative control (KN), positive control (KP, 9 mg paracetamol/200g BW), and three treatment groups (P1, P2, and P3). They received PAE orally at 317.5, 635, and 1270 mg/kg BW post-DPT vaccination. Pre and post-treatment rectal temperatures were recorded. After the study, serum TNF-α and IL-10 levels were assessed via ELISA. Data underwent ANOVA and Pearson analysis. Results: PAE significantly lowered rectal temperature and TNF-α and IL-10 levels (p