Evaluation of Total Phenolic Content and Antioxidant Activity of Carica papaya Seeds: Optimization and FTIR Analysis

Background: Medicinal plants are rich sources of antioxidants, which help combat oxidative stress in the body by neutralizing harmful free radicals. Carica papaya is an herbaceous, annual plant and it belongs to Caricaceae family. It shows various phytochemicals and acts as biological properties. Methods: In this study, seed extract from Carica papaya was used to increase total phenolic content through extraction procedure involves optimizing physico-chemical parameters and Soxhlet extraction process. Finally studied antioxidant activity by reducing power assay. Result: Total phenolic content (TPC) is extracted using a variety of solvents, solvent concentrations (%), extraction time (t), pH and particle mesh sizes (microns). The ideal conditions in this case are methanol, 80% solvent, 24 hours, pH-6 and 120 mesh size. In this extraction, the TPC content was found to be 27.83+0.28 µg GAE/ml. Similarly, the TPC concentration was enhanced during the Soxhlet extraction procedure, reaching 36.16+0.28 µg GAE/ml. That sample was used for antioxidant research following Soxhlet extraction process. The results revealed a reducing power ability of 79.81+0.22% at the highest concentration of 2000 µg/ml and additionally, the IC50 values were found to be concentration-dependent, with a value of 92.17 µg/ml for reducing power ability.