Development of Primary Cell Culture from Different Tissues of Osteobrama belangeri, a Near Threatened Fish Species of the North-Eastern Region of India

Background: Primary cell culture closely matches the physiological and biochemical features of an in vivo system and it serves as a representative model for studying many key issues of disease, reproduction, genetics and biotechnology. The present study aimed to develop a primary culture system from the Gill, Liver and Kidney tissues of Osteobrama belangeri, which is an important candidate species for aquaculture. Methods: The primary culture was established from tissues through the explant method. The cultured cells were maintained in Leibovitz’s L-15 Medium supplemented with FBS (Fetal Bovine Serum) and Antibiotic Antimycotic Solution 100X Liquid. The growth optimization of cultured cells was performed at different incubation temperatures (25°C, 28°C, 30°C) and FBS concentrations (5%, 10% and 15%). Result: The maximum growth rate of cultured gill cells was recorded at 30°C temperature and 15% FBS. The cultured cells were characterized for species authentication using DNA barcoding. The sequence analysis reveals and confirms the species of origin. This primary culture of different tissues provides a foundational in vitro platform for conservation genetics, disease diagnostics and biotechnological applications in O. belangeri.