[Effect of yeast extract induction on carbohydrate components of Sorbus aucuparia].

Carbohydrates are involved in energy and material metabolism, playing an essential role in plant growth, development, and stress response. This study used Sorbus aucuparia suspension cells(SASCs) as a model to investigate the effects of yeast extract(YE) induction on the carbohydrate components of SASCs. Polysaccharides were extracted using an orbital shaker, and total and soluble sugar content was determined. The types and quantities of oligosaccharides were analyzed by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) and ultra-performance liquid chromatography-evaporative light scattering detector(UPLC-ELSD), and the monosaccharide composition of the crude polysaccharides of SASCs was also examined. The results showed that YE induction did not affect the total sugar and soluble sugar content in SASCs. The types of oligosaccharides in SASCs were limited, with only sucrose and kestose detected. Under YE induction, the content of kestose decreased. There were no differences in the monosaccharide composition of the crude polysaccharides from YE-induced SASCs, while the content of D-fructose, D-mannitol, D-allose, and D-glucose increased, and the content of D-galacturonic decreased. These findings suggested that changes in carbohydrate components varied with stress factors. YE induction had a relatively minor effect on the metabolism of carbohydrate substances in SASCs but significantly affects the formation of characteristic biphenyl and dibenzofuran-type phytoalexins in the subfamily Maloideae.