[Therapeutic effects and mechanisms of Huangqi Chifeng Decoction on hyperlipidemia in rats: an integrated metabolomics approach].

This study aimed to evaluate the lipid-lowering effect of Huangqi Chifeng Decoction(HQCFD) using a hyperlipidemia rat model and investigated the alterations in endogenous metabolites and underlying mechanisms based on a serum metabolomics approach employing gas chromatography-mass spectrometry(GC-MS). A hyperlipidemia rat model was established by feeding a high-fat diet, and after successful model induction, rats were administered HQCFD by gavage for four weeks. By detecting the four blood lipid profiles, organ indexes, and histopathological changes in liver tissue, the regulatory effect of HQCFD on hyperlipidemia was assessed. GC-MS was applied for non-targeted metabolomic analysis of serum samples from rats in each group to screen potential biomarkers, followed by enrichment of related metabolic pathways to explore the potential mechanism of action. The results showed that after four weeks of high-fat diet feeding, compared with the normal diet group, the high-fat diet group exhibited significantly increased levels of total cholesterol(TC), triglycerides(TG), and low-density lipoprotein cholesterol(LDL-C)(P