Association Between β-ketoacyl-acyl Carrier Protein Synthase Genes and Seed Oil Accumulation in Jatropha curcas L.

Jatropha curcas L. is a bioenergy crop of interest because of the high oil content in its seeds suitable for conversion into biofuels. However, its oil content is extremely variable among accessions and the mechanism of oil accumulation is poorly understood in this oleaginous species. In this study we analyzed cloned plants of three chemotypes of J. curcas collected in Chiapas, Mexico: CAC-3, COM-1 and MAP-2. All are monoecious and accumulating different amounts of seed oil: 10 %, 30 % and 54 %. We studied the expression of the β-ketoacyl-acyl carrier protein (ACP) synthase genes (KAS) in developing seeds and their relationship with the content and composition of the oil. Differences of the levels of expression of the KASI and KASII genes were found, while KASIII gene was expressed at high levels in all three chemotypes. The expression of KASI and KASII was statistically associated to the oil accumulation. Results of this study are discussed based on the regulation of the transcription of the KAS genes, in order to contribute to the understanding of the oil accumulation in the seed and could be of value for designing biotechnological strategies with which to improve this species.