Anti-prostate cancer activity and mechanism of the sesquiterpenoid lactone THA from Cyanthillium cinereum

Abstract Purpose This study aims to evaluate the in vitro effect of 8α-tigloyloxyhirsutinolide-13-O-acetate (THA) on human prostate cancer cells and to examine its in vivo inhibitory effect on tumor growth in a mouse xenograft model. Methods In this study, the androgen-independent prostate cancer (AIPC) cell line PC-3 was employed. To investigate the effects of THA, we assessed cell proliferation, migration, invasion, apoptosis, and cell cycle distribution using MTT, wound healing, Transwell invasion, colony formation assays, Hoechst-33342 staining and flow cytometry. A mouse xenograft tumor model was established, and treatment groups received THA. The expression levels of p-STAT3, p-AKT, p-Erk1/2, CDK2, cyclin E1, MMP9, Bcl-2, Bax, and survivin were analyzed by Western blotting. Tumor formation and progression were evaluated by monitoring tumor volume. Results Our study showed that THA strongly inhibited cell growth and induced apoptosis, as well as caused cell cycle arrest at the S phase in PC-3 cells. THA also strongly suppressed cell migration and invasion, and decreased spheroid and colony formation. Animal experiment showed that THA inhibited the growth of PC-3 xenograft tumor in nude mice. Studies on mechanisms of actions showed that the potent anti-prostate cancer effects of THA were associated with inhibition of STAT3, AKT, and Erk1/2 pathways. Conclusion Our findings demonstrate that the potent anti-prostate cancer activity of THA is associated with inhibition of the STAT3, AKT, and Erk1/2 pathways. These data lend support to the further investigation of THA as a potential therapeutic agent against prostate cancer.