BeamMap: Beam Enabled Accurate Mapping and Molecular Analyte Profiling for topography and biochemistry spatial correlation

Abstract BeamMap is a multimodal bioimaging instrument that integrates scanning electron microscopy with vacuum electrospray-enabled mass spectrometry to enable in situ correlation of biosample structure and untargeted chemistry. It has potential to achieve subcellular topographical imaging alongside chemical mapping via vacuum nanoelectrospray soft ionization with tunable energy for broad range of analyte detection. Demonstrated on a model imaging system, the workflow links morphology and molecular analysis concurrently within the same analytical environment. The BeamMap approach aims to expand discovery-driven single-cell and multiomics investigations across diverse biological samples and tissues.