Random barcode transposon-site sequencing of Rhodopseudomonas palustris CGA009

Research Square 2026 Preprint Open access

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Authors: Yasuhiro Oda, Valentine V. Trotter, Catalina Vega Hurtado, Benjamin Weinberg, Hualan Liu, Adity Biswas, Ying-Chih Chuang, Nicholas W. Haas, José P. M. Heerdink-Santos, Nathan M. Lewis, Nicole Marquez Reyes, Brittany E. Mazny
+5 more Olivia F Schakel, James B. McKinlay, Caroline S. Harwood, Adam M. Deutschbauer, Kathryn R. Fixen
DOI: 10.21203/rs.3.rs-9430391/v1
Cited by: 1
References: 7

Abstract

Abstract Rhodopseudomonas palustris is one of the most metabolically versatile organisms ever described and is of interest for a variety of biotechnology applications. Here we describe a random barcoded transposon (Tn) insertion library in the strain CGA009 along with gene fitness analyses from 208 experiments spanning 54 growth conditions.

Fields of Study & Subjects

Microbial Community Ecology and Physiology Algal biology and biofuel production Microbial Metabolic Engineering and Bioproduction

Publication details

Journal / source
Research Square
Publisher
Springer Science and Business Media LLC
Publication date
20 April 2026
Document type
Preprint