Prevalence of Acinetobacter spp. and Antibiotic Susceptibility Pattern in Various Clinical Samples in a Tertiary Care Hospital

Background: Acinetobacter spp.are opportunistic gram-negative pathogens responsible for severe hospital-acquired infections, particularly in immunocompromised and device-dependent patients.A. baumannii is the most clinically significant species, exhibiting remarkable environmental persistence and extensive antimicrobial resistance, including carbapenem resistance.Its diverse resistance mechanisms drive multidrug resistance, making treatment challenging and heightening morbidity, especially in ICU settings, thereby necessitating continuous surveillance and susceptibility profiling.Methods: A retrospective study (January-June 2025) was conducted in the Microbiology Laboratory, D. Y. Patil University, Navi Mumbai.Clinical samples (urine, CSF, pus, pleural fluid, endotracheal secretions, BAL) were processed, and Acinetobacter spp.were identified using standard methods; antimicrobial susceptibility testing was performed according to CLSI guidelines.Result: During the study period, a total of 2,582 clinical samples were processed, of which 69 (2.67%) yielded Acinetobacter spp.Isolates.The highest prevalence was observed in endotracheal (ET) secretions (28.1%, 9/32).This indicates that Acinetobacter spp. was most frequently isolated from respiratory specimens.Across all specimens, colistin showed 100% sensitivity, making it the most effective drug.In urine isolates, the highest susceptibility was to colistin (100%).CSF isolates demonstrated 50% sensitivity to cefepime and carbapenems.Pus isolates showed the best response to colistin (100%). Conclusion:The study concluded that the prevalence of Acinetobacter spp.Varies considerably across clinical specimens, with the highest detection observed in endotracheal aspirates and markedly lower rates in urine, cerebrospinal fluid, and pleural fluid samples.