Preliminary Screening of Traditional Medicine Against Porcine Reproductive and Respiratory Syndrome Virus

Objective: Due to the lack of specific anti-PRRSV drugs and limited cross-protection of the vaccine, PRRSV outbreaks cause substantial economic and social losses. This study aimed to identify anti-PRRSV agents from traditional Chinese medicines and predict their antiviral mechanisms. Methods: Screen anti-PRRSV agents by CCK-8/RT-qPCR assays and predict their mechanisms using network pharmacology. Results: Resveratrol, berberine, puerarin, Portulaca oleracea water extract, and vanillic acid exhibited anti-PRRSV activity. Their potential molecular targets/mechanisms were predicted via network pharmacology. Conclusion: Five anti- PRRSV agents were screened, their targets and mechanisms elucidated, providing a theoretical basis for the development of PRRSV drugs and further studies.