IMMUNOGLOBULIN AND SOME CYTOKINES TITER IN BOVINE MILK OBTAINED FROM CLINICAL AND SUBCLINICAL MASTITIS

Mastitis is the most frequent and highly economically damaging disease of dairy cows. According to signs, it is classified into clinical, subclinical, and chronic mastitis. Immune response strategies differ according to the form of mastitis. Somatic cell count in the produced milk is the most important criterion of these forms. The current study aims to find immune response strategies for clinical and subclinical bovine mastitis. For this purpose, milk samples from 60 adult dairy cows (10 cows were clinically infected while 50 were apparently healthy) were collected. IgG, IgM, IL-6, IL-10, and IL-12 were determined in the collected milk samples using an ELISA test. According to the California Mastitis test, subclinical mastitis was recorded at a rate of 68%, and then the milk samples were classified into clinical mastitic, subclinical mastitic, and healthy milk. Results of the ELISA test showed that the highest IgM and IgG concentrations were recorded in clinical and subclinical mastitic milk, respectively. The highest concentrations of IL-6 and IL-10 were recorded in milk from subclinical mastitis cases, while the highest IL-12 concentrations were recorded in milk from clinical mastitis.